Integrated Full-Length Transcriptome and Metabolome Profiling Reveals Flavonoid Regulation in Response to Freezing Stress in Potato

Cold stress impairs plant growth and development, resulting in crop failure. Cultivated potato (Solanum tuberosum L.) is sensitive to freezing, while its wild relative, S. commersonii, has a strong freezing tolerance. To decipher the anti-freezing mechanism of CM, we carried out transcriptomic metabolomic analysis an variety CM (a type commersonii) freeze-sensitive DM Solanum L.). A total 49,232 high-quality transcripts from 12,811 gene loci, including 46,772 coding sequences 2018 non-coding RNAs, were identified. KEEG enrichment differentially expressed genes (DEGs) between two varieties showed that flavonoid biosynthesis pathway was strongly induced by stress, which proven metabolome analysis. Consistent with accumulation more flavonoids, nearly all significantly upregulated than those DM. The transcript levels chalcone synthase (CHS-1) isoforms four 3′-hydroxylase (F3′H-1) confirmed qRT-PCR. Co-expression identified one Myb-related three UGTs (UDP-glycosyltransferase) during stress. Our findings support enhanced greater ofglycosylatedflavonoids resistant types types, maybe accounting for increased tolerance freeze-resistant varieties.